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tetracycline repressor vp16 transactivator fusion protein  (Addgene inc)


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    Addgene inc tetracycline repressor vp16 transactivator fusion protein
    Tetracycline Repressor Vp16 Transactivator Fusion Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+cmv+rtta3+blast/pLenti+CMV+rtTA3+Blast+(w756-1)+(Plasmid+%2326429)/bio_rxiv__64898__2026__10__14__759247-39-8-18
    Average 94 stars, based on 123 article reviews
    tetracycline repressor vp16 transactivator fusion protein - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Combinational Inhibition of MEK and AKT Synergistically Induces Melanoma Stem Cell Apoptosis and Blocks NRAS Tumor Growth
    Article Snippet: .. To generate Dox-inducible lentivirus that can induce CD133 expression, HEK293FT packaging cells were co-transfected with pLenti-CMV-rtTA3 Blast (Addgene, Watertown, MA, USA) and psPAX2 and pMD2.G (Addgene), with or without pLV-EGFP/Neo-TRE3GCD133 (VectorBuilder Inc., Chicago, IL, USA) using Lipofectamine LTX (ThermoFisher Sci, Waltham, MA, USA). ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1), a gift from E. Campeau (Addgene plasmid no. 26429), followed by selection in medium containing 10 μg/mL blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis.
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml−1 blasticidin. ..

    Article Title: Combinational Inhibition of MEK and AKT Synergistically Induces Melanoma Stem Cell Apoptosis and Blocks NRAS Tumor Growth
    Article Snippet: .. To generate Dox-inducible lentivirus that can induce CD133 expression, HEK293FT packaging cells were co-transfected with pLenti-CMV-rtTA3 Blast (Addgene, Watertown, MA, USA) and psPAX2 and pMD2.G (Addgene), with or without pLV-EGFP/Neo-TRE3G-CD133 (VectorBuilder Inc., Chicago, IL, USA) using Lipofectamine LTX (ThermoFisher Sci, Waltham, MA, USA). ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml −1 blasticidin. ..

    Transduction:

    Article Title: High-resolution promoter interaction analysis implicates genes involved in the activation of Type 3 Innate Lymphoid Cells in autoimmune disease risk
    Article Snippet: .. In brief, MNK-3 cells were transduced with lentivirus containing pLenti CMV rtTA3 Blast (Addgene #26429), selected by blasticidin S, and then infected with TRE3G-dCas9-KRAB-P2A-mCherry lentivirus. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1), a gift from E. Campeau (Addgene plasmid no. 26429), followed by selection in medium containing 10 μg/mL blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis.
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml−1 blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml −1 blasticidin. ..

    Article Title: CRISPRi Screening Identifies SON and MAP4K1 as Regulators of Type III Cytokine Expression in Innate Lymphoid Cells
    Article Snippet: .. In brief, MNK3 cells were transduced with pLenti CMV rtTA3 Blast (Addgene plasmid #26429), selected with 10 μg/ml Blasticidin S HCL (Gibco), transduced with a TRE3G-dCas9-KRAB-P2A-mCherry lentivirus, and subcloned ( ). ..

    Infection:

    Article Title: High-resolution promoter interaction analysis implicates genes involved in the activation of Type 3 Innate Lymphoid Cells in autoimmune disease risk
    Article Snippet: .. In brief, MNK-3 cells were transduced with lentivirus containing pLenti CMV rtTA3 Blast (Addgene #26429), selected by blasticidin S, and then infected with TRE3G-dCas9-KRAB-P2A-mCherry lentivirus. ..

    Generated:

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1), a gift from E. Campeau (Addgene plasmid no. 26429), followed by selection in medium containing 10 μg/mL blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis.
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml−1 blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml −1 blasticidin. ..

    Article Title: Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress
    Article Snippet: .. For Dsup-EGFP construct, the DNA fragment encoding Dsup was amplified using primers 5’- TCA AGC TTC GAA TTC TGC AGT CGA CAT GGC ATC CAC ACA CCA ATC - 3’ and 5’ – TCA CCA TGG TGG CGA CCG GTG GAT CCT TCC TCT TCC GTC CTC CAG - 3’, and replaced H2B in H2B-EGFP-N1 by Gibson Assembly. pLenti-CMV-rtTA3-Blast (#26429), psPAX2 (#12260) and pMD2.G (#12259) were ordered from Addgene. pLenti-TetO-EGFP-Puro construct was generated previously. ..

    Plasmid Preparation:

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1), a gift from E. Campeau (Addgene plasmid no. 26429), followed by selection in medium containing 10 μg/mL blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis.
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml−1 blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml −1 blasticidin. ..

    Article Title: CRISPRi Screening Identifies SON and MAP4K1 as Regulators of Type III Cytokine Expression in Innate Lymphoid Cells
    Article Snippet: .. In brief, MNK3 cells were transduced with pLenti CMV rtTA3 Blast (Addgene plasmid #26429), selected with 10 μg/ml Blasticidin S HCL (Gibco), transduced with a TRE3G-dCas9-KRAB-P2A-mCherry lentivirus, and subcloned ( ). ..

    Selection:

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1), a gift from E. Campeau (Addgene plasmid no. 26429), followed by selection in medium containing 10 μg/mL blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis.
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml−1 blasticidin. ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with pLenti CMV rtTA3 Blast (w756-1; a gift from E. Campeau; Addgene, plasmid 26429), followed by selection in medium containing 10 μg ml −1 blasticidin. ..

    Construct:

    Article Title: Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress
    Article Snippet: .. For Dsup-EGFP construct, the DNA fragment encoding Dsup was amplified using primers 5’- TCA AGC TTC GAA TTC TGC AGT CGA CAT GGC ATC CAC ACA CCA ATC - 3’ and 5’ – TCA CCA TGG TGG CGA CCG GTG GAT CCT TCC TCT TCC GTC CTC CAG - 3’, and replaced H2B in H2B-EGFP-N1 by Gibson Assembly. pLenti-CMV-rtTA3-Blast (#26429), psPAX2 (#12260) and pMD2.G (#12259) were ordered from Addgene. pLenti-TetO-EGFP-Puro construct was generated previously. ..

    Amplification:

    Article Title: Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress
    Article Snippet: .. For Dsup-EGFP construct, the DNA fragment encoding Dsup was amplified using primers 5’- TCA AGC TTC GAA TTC TGC AGT CGA CAT GGC ATC CAC ACA CCA ATC - 3’ and 5’ – TCA CCA TGG TGG CGA CCG GTG GAT CCT TCC TCT TCC GTC CTC CAG - 3’, and replaced H2B in H2B-EGFP-N1 by Gibson Assembly. pLenti-CMV-rtTA3-Blast (#26429), psPAX2 (#12260) and pMD2.G (#12259) were ordered from Addgene. pLenti-TetO-EGFP-Puro construct was generated previously. ..



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