tetracycline repressor vp16 transactivator fusion protein (Addgene inc)
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Addgene inc
tetracycline repressor vp16 transactivator fusion protein
Tetracycline Repressor Vp16 Transactivator Fusion Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv+rtta3+blast/pLenti+CMV+rtTA3+Blast+(w756-1)+(Plasmid+%2326429)/bio_rxiv__64898__2026__10__14__759247-39-8-18
Average 94 stars, based on 123 article reviews
Tetracycline Repressor Vp16 Transactivator Fusion Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv+rtta3+blast/pLenti+CMV+rtTA3+Blast+(w756-1)+(Plasmid+%2326429)/bio_rxiv__64898__2026__10__14__759247-39-8-18
Average 94 stars, based on 123 article reviews
tetracycline repressor vp16 transactivator fusion protein - by Bioz Stars,
2026-11
94/100 stars
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Expressing:Article Title: Combinational Inhibition of MEK and AKT Synergistically Induces Melanoma Stem Cell Apoptosis and Blocks NRAS Tumor Growth Article Snippet: .. To generate Dox-inducible lentivirus that can induce CD133 expression, HEK293FT packaging cells were co-transfected with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with Article Title: Combinational Inhibition of MEK and AKT Synergistically Induces Melanoma Stem Cell Apoptosis and Blocks NRAS Tumor Growth Article Snippet: .. To generate Dox-inducible lentivirus that can induce CD133 expression, HEK293FT packaging cells were co-transfected with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Transduction:Article Title: High-resolution promoter interaction analysis implicates genes involved in the activation of Type 3 Innate Lymphoid Cells in autoimmune disease risk Article Snippet: .. In brief, MNK-3 cells were transduced with lentivirus containing Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: CRISPRi Screening Identifies SON and MAP4K1 as Regulators of Type III Cytokine Expression in Innate Lymphoid Cells Article Snippet: .. In brief, MNK3 cells were transduced with Infection:Article Title: High-resolution promoter interaction analysis implicates genes involved in the activation of Type 3 Innate Lymphoid Cells in autoimmune disease risk Article Snippet: .. In brief, MNK-3 cells were transduced with lentivirus containing Generated:Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress Article Snippet: .. For Dsup-EGFP construct, the DNA fragment encoding Dsup was amplified using primers 5’- TCA AGC TTC GAA TTC TGC AGT CGA CAT GGC ATC CAC ACA CCA ATC - 3’ and 5’ – TCA CCA TGG TGG CGA CCG GTG GAT CCT TCC TCT TCC GTC CTC CAG - 3’, and replaced H2B in H2B-EGFP-N1 by Gibson Assembly. Plasmid Preparation:Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: CRISPRi Screening Identifies SON and MAP4K1 as Regulators of Type III Cytokine Expression in Innate Lymphoid Cells Article Snippet: .. In brief, MNK3 cells were transduced with Selection:Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene plasmid no. 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg/mL puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml−1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1KO cells with Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: Briefly, U-2 OS cells were transfected with px459 (Addgene, plasmid 48139) encoding FSP1 sgRNA guide 2, followed by selection in medium containing 1 μg ml −1 puromycin and isolation of individual clones using cloning rings. .. Expression lines were generated by transduction of U-2 OS FSP1 KO cells with Construct:Article Title: Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress Article Snippet: .. For Dsup-EGFP construct, the DNA fragment encoding Dsup was amplified using primers 5’- TCA AGC TTC GAA TTC TGC AGT CGA CAT GGC ATC CAC ACA CCA ATC - 3’ and 5’ – TCA CCA TGG TGG CGA CCG GTG GAT CCT TCC TCT TCC GTC CTC CAG - 3’, and replaced H2B in H2B-EGFP-N1 by Gibson Assembly. Amplification:Article Title: Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress Article Snippet: .. For Dsup-EGFP construct, the DNA fragment encoding Dsup was amplified using primers 5’- TCA AGC TTC GAA TTC TGC AGT CGA CAT GGC ATC CAC ACA CCA ATC - 3’ and 5’ – TCA CCA TGG TGG CGA CCG GTG GAT CCT TCC TCT TCC GTC CTC CAG - 3’, and replaced H2B in H2B-EGFP-N1 by Gibson Assembly. |